Journal
NATURE METHODS
Volume 5, Issue 2, Pages 163-165Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH1170
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Funding
- NCI NIH HHS [R01 CA127590, U54 CA116867, R01 CA127590-01A1, U54CA116867, CA127590, K22 CA103843] Funding Source: Medline
- NICHD NIH HHS [R01 HD056369] Funding Source: Medline
- PHS HHS [KCA103843A, RHD056369A] Funding Source: Medline
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We developed a strategy to introduce epitope tag-encoding DNA into endogenous loci by homologous recombination-mediated 'knock-in'. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines, and can facilitate many functional analyses including western blot, immunoprecipitation, immunofluorescence and chromatin immunoprecipitation-microarray (ChIP-chip). The knock-in approach provides a general solution for the study of proteins to which antibodies are substandard or not available.
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