Journal
NATURE METHODS
Volume 5, Issue 6, Pages 527-529Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1211
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Funding
- NCI NIH HHS [P30 CA034196] Funding Source: Medline
- NIAID NIH HHS [K25 AI065459] Funding Source: Medline
- PHS HHS [K25-65459] Funding Source: Medline
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Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.
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