Journal
NATURE METHODS
Volume 5, Issue 5, Pages 401-403Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1207
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Fluorescence resonance energy transfer (FRET) with fluorescent proteins is a powerful method for detection of protein-protein interactions, enzyme activities and small molecules in the intracellular milieu. Aided by a new violet-excitable yellow-fluorescing variant of Aequorea victoria GFP, we developed dual FRET-based caspase-3 biosensors. Owing to their distinct excitation profiles, each FRET biosensor can be ratiometrically imaged in the presence of the other.
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