Journal
NATURE METHODS
Volume 5, Issue 9, Pages 777-779Publisher
NATURE RESEARCH
DOI: 10.1038/NMETH.1244
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Funding
- Center for Excellence in Genome Sciences
- Canadian Institutes of Health Research Fellowship
- US National Institutes of Health Ruth L. Kirschstein Fellowship
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Here we report a highly efficient and simplified strategy to preadenylate bar-coded oligonucleotides designed for microRNA (miRNA) capture and multiplex analysis. Using this approach, we enzymatically preadenylated bar-coded oligonucleotides with high efficiency when compared to the chemical method currently used by miRNA investigators. As a case study, we used these oligonucleotides in an ATP-independent ligation to miRNAs, suggesting the utility of our method in end-capture protocols and high-throughput sequencing applications.
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