Journal
NATURE METHODS
Volume 5, Issue 7, Pages 601-603Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1225
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Current techniques for making transgenic mice are cumbersome, requiring trained personnel, costly infrastructure and collection of many zygotes from mice that are then killed. We developed a reproducible nonterminal technique for transfecting genes in undifferentiated spermatogonia through in vivo electroporation of the testis; about 94% of male mice electroporated with different transgenes successfully sired transgenic pups. Such electroporated males provide a valuable resource for continuous production of transgenic founders for more than a year.
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