Journal
NATURE MEDICINE
Volume 17, Issue 2, Pages 211-U105Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nm.2284
Keywords
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Funding
- US National Institutes of Health [R01-AG023374, R01-ES015888, R21-ES015893, R21-CA150009]
- US Department of Defense [W81XWH-07-1-0616, W81XWH-08-1-0472]
- Elsa Pardee Foundation
- M. D. Anderson Cancer Center [CCSG-5 P30 CA016672-34, ES007784]
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Cancer stem cells (CSCs), or tumor-initiating cells, are involved in tumor progression and metastasis(1). MicroRNAs (miRNAs) regulate both normal stem cells and CSCs2-5, and dysregulation of miRNAs has been implicated in tumorigenesis(6). CSCs in many tumors-including cancers of the breast(7), pancreas(8), head and neck(9), colon(10,11), small intestine(12), liver(13), stomach(14), bladder(15) and ovary(16)-have been identified using the adhesion molecule CD44, either individually or in combination with other marker(s). Prostate CSCs with enhanced clonogenic(17) and tumor-initiating and metastatic(18,19) capacities are enriched in the CD44(+) cell population, but whether miRNAs regulate CD44(+) prostate cancer cells and prostate cancer metastasis remains unclear. Here we show, through expression analysis, that miR-34a, a p53 target(20-24), was underexpressed in CD44(+) prostate cancer cells purified from xenograft and primary tumors. Enforced expression of miR-34a in bulk or purified CD44(+) prostate cancer cells inhibited clonogenic expansion, tumor regeneration, and metastasis. In contrast, expression of miR-34a antagomirs in CD44(-) prostate cancer cells promoted tumor development and metastasis. Systemically delivered miR-34a inhibited prostate cancer metastasis and extended survival of tumor-bearing mice. We identified and validated CD44 as a direct and functional target of miR-34a and found that CD44 knockdown phenocopied miR-34a overexpression in inhibiting prostate cancer regeneration and metastasis. Our study shows that miR-34a is a key negative regulator of CD44(+) prostate cancer cells and establishes a strong rationale for developing miR-34a as a novel therapeutic agent against prostate CSCs.
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