4.7 Article

Technical considerations for functional sequencing assays

Journal

NATURE IMMUNOLOGY
Volume 13, Issue 9, Pages 802-807

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NATURE PORTFOLIO
DOI: 10.1038/ni.2407

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Next-generation sequencing technologies need careful design of experiments and evaluation of results to meet field requirements. Here we discuss technical considerations for these high-throughput assays, together with criteria to assess the quality of the results and the necessary validation.

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