Journal
NATURE IMMUNOLOGY
Volume 10, Issue 6, Pages 655-U131Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ni.1735
Keywords
-
Categories
Funding
- Wellcome Trust [085096]
- New York University School of Medicine
- US National Institutes of Health [R37 AI32524, R01 GM086852A, R01 CA125195, RO1 AI050737]
- Pew Scholars Program
- Cancer Research Institute
- Boehringer Ingelheim
- Genome Research in Austria
- Howard Hughes Medical Institute
Ask authors/readers for more resources
Coordinated recombination of homologous antigen receptor loci is thought to be important for allelic exclusion. Here we show that homologous immunoglobulin alleles pair in a stage-specific way that mirrors the recombination patterns of these loci. The frequency of homologous immunoglobulin pairing was much lower in the absence of the RAG-1-RAG-2 recombinase and was restored in Rag1(-/-) developing B cells with a transgene expressing a RAG-1 active-site mutant that supported DNA binding but not cleavage. The introduction of DNA breaks on one immunoglobulin allele induced ATM-dependent repositioning of the other allele to pericentromeric heterochromatin. ATM activated by the cleaved allele acts in trans on the uncleaved allele to prevent biallelic recombination and chromosome breaks or translocations.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available