Journal
NATURE IMMUNOLOGY
Volume 11, Issue 1, Pages 90-U106Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ni.1832
Keywords
-
Categories
Funding
- US National Institutes of Health [AI 55277]
- US National Science Foundation
- Howard Hughes Medical Institute
- Human Frontier Science Program
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [U19AI057229, R01AI022511, N01AI055277] Funding Source: NIH RePORTER
Ask authors/readers for more resources
The organization and dynamics of receptors and other molecules in the plasma membrane are not well understood. Here we analyzed the spatio-temporal dynamics of T cell antigen receptor (TCR) complexes and linker for activation of T cells (Lat), a key adaptor molecule in the TCR signaling pathway, in T cell membranes using high-speed photoactivated localization microscopy, dual-color fluorescence cross-correlation spectroscopy and transmission electron microscopy. In quiescent T cells, both molecules existed in separate membrane domains (protein islands), and these domains concatenated after T cell activation. These concatemers were identical to signaling microclusters, a prominent hallmark of T cell activation. This separation versus physical juxtapositioning of receptor domains and domains containing downstream signaling molecules in quiescent versus activated T cells may be a general feature of plasma membrane-associated signal transduction.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available