Journal
NATURE GENETICS
Volume 43, Issue 3, Pages 220-U65Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ng.765
Keywords
-
Categories
Funding
- la Ligue Nationale contre le Cancer (Equipe labellisee la Ligue)
- Curie Programme Incitatif et Collaboratif (PIC)
- European Network of Excellence Epigenome [LSHG-CT-2004-503433]
- ACI-2007-Canceropole IdF 'Breast cancer and Epigenetics'
- Agence Nationale de la Recherche (ANR) 'EcenS' [ANR-09-BLAN-0257-01]
- INCa 'GepiG'
- European Research Council (ERC) [2009-AdG-20090506]
- le Canceropole Ile-de-France
- l'INCA
Ask authors/readers for more resources
HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of long nuclear noncoding transcripts corresponding to major satellite repeats at the periphery of pericentric heterochromatin. Furthermore, we find that major transcripts in the forward orientation specifically associate with SUMO-modified HP1 proteins. We identified this modification as SUMO-1 and mapped it in the hinge domain of HP1 alpha. Notably, the hinge domain and its SUMOylation proved critical to promote the initial targeting of HP1a to pericentric domains using de novo localization assays, whereas they are dispensable for maintenance of HP1 domains. We propose that SUMO-HP1, through a specific association with major forward transcript, is guided at the pericentric heterochromatin domain to seed further HP1 localization.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available