Journal
NATURE CHEMISTRY
Volume 4, Issue 8, Pages 621-628Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEM.1397
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Funding
- ILL/ESRF studentship
- Chemistry Department, University of Reading
- Diamond Light Source
- University of Reading University studentship
- Royal Society
- Royal Irish Academy
- Science Foundation Ireland [06/RF/CHP035]
- Biotechnology and Biological Sciences Research Council [B19997] Funding Source: researchfish
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The ruthenium complex [Ru(phen)(2)(dppz)](2+) (where phen is phenanthroline and dppz dipyridophenazine is known as a 'light switch' complex because its luminescence in solution is significantly enhanced in the presence of DNA. This property is poised to serve in diagnostic and therapeutic applications, but its binding mode with DNA needs to be elucidated further. Here, we describe the crystal structures of the Lambda enantiomer bound to two oligonucleotide duplexes. The dppz ligand intercalates symmetrically and perpendicularly from the minor groove of the d(CCGGTACCGG)(2) duplex at the central TA/TA step, but not at the central AT/AT step of d(CCGGATCCGG)(2). In both structures, however, a second ruthenium complex links the duplexes through the combination of a shallower angled intercalation into the C1C2/G(9)G(10) step at the end of the duplex, and semi-intercalation into the G(3)G(4) step of an adjacent duplex. The TA/TA specificity of the perpendicular intercalation arises from the packing of phenanthroline ligands against the adenosine residue.
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