Journal
NATURE CHEMISTRY
Volume 2, Issue 3, Pages 179-186Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEM.545
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Funding
- US Department of Energy [DE-FG02-07ER15892]
- National Center for Research Resources of the National Institutes of Health [1R21-RR023149]
- U.S. Department of Energy (DOE) [DE-FG02-07ER15892] Funding Source: U.S. Department of Energy (DOE)
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Observing the dynamics of single biomolecules over prolonged time periods is difficult to achieve without significantly altering the molecule through immobilization. It can, however, be accomplished using the anti-Brownian electrokinetic trap, which allows extended investigation of solution-phase biomolecules-without immobilization-through real-time electrokinetic feedback. Here we apply the trap to study an important photosynthetic antenna protein, allophycocyanin. The technique allows the observation of single molecules of solution-phase allophycocyanin for more than one second. We observe a complex relationship between fluorescence intensity and lifetime that cannot be explained by simple static spontaneous emission lifetime, which is typically assumed to be constant. Our methods provide a new window into the dynamics of fluorescent proteins and the observations are relevant for the interpretation of in vivo single-molecule imaging experiments, bacterial photosynthetic regulation and biomaterials for solar energy harvesting.
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