Journal
NATURE CHEMICAL BIOLOGY
Volume 10, Issue 7, Pages 512-523Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEMBIO.1556
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Funding
- NIGMS NIH HHS [T32 GM065103] Funding Source: Medline
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Synergistic advances in optical physics, probe design, molecular biology, labeling techniques and computational analysis have propelled fluorescence imaging into new realms of spatiotemporal resolution and sensitivity. This review aims to discuss advances in fluorescent probes and live-cell labeling strategies, two areas that remain pivotal for future advances in imaging technology. Fluorescent protein- and bio-orthogonal-based methods for protein and RNA imaging are discussed as well as emerging bioengineering techniques that enable their expression at specific genomic loci (for example, CRISPR and TALENs). Important attributes that contribute to the success of each technique are emphasized, providing a guideline for future advances in dynamic live-cell imaging.
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