Journal
NATURE CHEMICAL BIOLOGY
Volume 10, Issue 9, Pages 739-744Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEMBIO.1587
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Funding
- Cancer Research UK [A11722]
- Biotechnology and Biological Sciences Research Council [B17092] Funding Source: researchfish
- Cancer Research UK [11722] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/J017094/1] Funding Source: researchfish
- EPSRC [EP/J017094/1] Funding Source: UKRI
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We present a crystal structure at 2.3-angstrom resolution of the recently described nucleolytic ribozyme twister. The RNA adopts a previously uncharacterized compact fold based on a double-pseudoknot structure, with the active site at its center. Eight highly conserved nucleobases stabilize the core of the ribozyme through the formation of one Watson-Crick and three noncanonical base pairs, and the highly conserved adenine 3' of the scissile phosphate is bound in the major groove of an adjacent pseudoknot. A strongly conserved guanine nucleobase directs its Watson-Crick edge toward the scissile phosphate in the crystal structure, and mechanistic evidence supports a role for this guanine as either a general base or acid in a concerted, general acid-base-catalyzed cleavage reaction.
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