Journal
NATURE CHEMICAL BIOLOGY
Volume 9, Issue 8, Pages 485-U33Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio.1278
Keywords
-
Categories
Funding
- Gordon and Betty Moore Foundation through the Caltech Programmable Molecular Technology Initiative
- US National Institutes of Health (NIH) [F32GM087102]
- University of North Carolina-Chapel Hill (UNC)
- NIH [1F32EB015846-01, RO1-DK019038]
Ask authors/readers for more resources
Whole-cell catalysts for non-natural chemical reactions will open new routes to sustainable production of chemicals. We designed a cytochrome 'P411' with unique serine-heme ligation that catalyzes efficient and selective olefin cyclopropanation in intact Escherichia coli cells. The mutation C400S in cytochrome P450(BM3) gives a signature ferrous CO Soret peak at 411 nm, abolishes monooxygenation activity, raises the resting-state Fe-III-to-Fe-II reduction potential and substantially improves NAD(P) H-driven activity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available