Journal
NATURE CHEMICAL BIOLOGY
Volume 6, Issue 4, Pages 264-266Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEMBIO.314
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Funding
- Swiss National Science Foundation [3100AQ-105541]
- EidgenOssische Technische Hochschule Zurich
- US National Institutes of Health [GM080374]
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We describe a new method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins.
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