Journal
NATURE CHEMICAL BIOLOGY
Volume 6, Issue 6, Pages 405-407Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio.352
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Funding
- Natural Sciences and Engineering Research Council of Canada
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We present a technique that uses (13)C NMR spectroscopy to measure kinetic isotope effects on the second-order rate constant (k(cat)/K(m)) for enzyme-catalyzed reactions. Using only milligram quantities of isotopically labeled substrates, precise competitive KIEs can be determined while following the ongoing reaction directly in a NMR spectrometer. Our results for the Vibrio cholerae sialidase-catalyzed hydrolysis of natural substrate analogs support a concerted enzymatic transition state for these reactions.
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