Journal
NATURE CHEMICAL BIOLOGY
Volume 5, Issue 10, Pages 715-717Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio.200
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Funding
- US National Institutes of Health [R21 DA020589, R01 GM62523]
- US Army Research Office Institute
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Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.
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