Journal
NATURE CHEMICAL BIOLOGY
Volume 5, Issue 10, Pages 718-720Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio.201
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Funding
- US National Institutes of Health [GM065966]
- US Defense Threat Reduction Agency [BRBAA08-l-2-0001]
- David and Lucile Packard Foundation
- US National Institutes of Health
- Ruth L. Kirschstein National Research Service Award [T32 GM070421]
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Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 10(8) (possibly as high as 10(14)). The new DNA catalysts require both Mn2+ and Zn2+, which is noteworthy because many natural DNA nucleases are bimetallic protein enzymes.
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