Journal
NATURE CELL BIOLOGY
Volume 20, Issue 10, Pages 1172-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41556-018-0199-8
Keywords
-
Categories
Funding
- Max Planck Society
- DFG [GRK1721, MI 1745/1]
- European Research Council [ERC-CoG-724209, ERC-StG-680241]
- EMBO Young Investigator award
- Boehringer Ingelheim Foundation Plus 3 Program grant
- DFG Emmy Noether Program [DFG JU 2957/1-1]
- Institut Curie
- CNRS
- [ANR-10-LBX-0038]
- [ANR-10-IDEX-0001-02 PSL]
- [ANR-12-BSV2-0007]
- [INCA 2013-1-PLBIO-02-ICR-1]
- [2014-PLBIO-11-ICR-1]
- [LISBOA-01-0145-FEDER-007660]
Ask authors/readers for more resources
Microtubules are central elements of the eukaryotic cytoskeleton that often function as part of branched networks. Current models for branching include nucleation of new microtubules from severed microtubule seeds or from. gamma-tubulin recruited to the side of a pre-existing microtubule. Here, we found that microtubules can be directly remodelled into branched structures by the microtubule-remodelling factor SSNA1 (also known as NA14 or DIP13). The branching activity of SSNA1 relies on its ability to self-assemble into fibrils in a head-to-tail fashion. SSNA1 fibrils guide protofilaments of a microtubule to split apart to form daughter microtubules. We further found that SSNA1 localizes at axon branching sites and has a key role in neuronal development. SSNA1 mutants that abolish microtubule branching in vitro also fail to promote axon development and branching when overexpressed in neurons. We have, therefore, discovered a mechanism for microtubule branching and implicated its role in neuronal development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available