Journal
NATURE CELL BIOLOGY
Volume 16, Issue 1, Pages 10-18Publisher
NATURE RESEARCH
DOI: 10.1038/ncb2895
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Funding
- Howard Hughes Medical Institute Funding Source: Medline
- NCI NIH HHS [P01 CA013106, P30 CA008748] Funding Source: Medline
- NIA NIH HHS [R01 AG016379] Funding Source: Medline
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RNA interference has become an indispensable tool for loss-of-function studies across eukaryotes. By enabling stable and reversible gene silencing, shRNAs provide a means to study long-term phenotypes, perform pool-based forward genetic screens and examine the consequences of temporary target inhibition in vivo. However, efficient implementation in vertebrate systems has been hindered by technical difficulties affecting potency and specificity. Focusing on these issues, we analyse current strategies to obtain maximal knockdown with minimal off-target effects.
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