Journal
NATURE CELL BIOLOGY
Volume 12, Issue 7, Pages 719-U212Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb2075
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Funding
- JSPS (Japan Society for the Promotion of Science)
- MEXT (Ministry of Education, Culture, Sports, Science and Technology) of Japan
- Inamori
- Matrix Science K.K. Foundations
- Uehara Memorial
- Naito
- Mochida Memorial
- Grants-in-Aid for Scientific Research [20114001, 21570196, 20114006] Funding Source: KAKEN
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Heterochromatin protein 1 (HP1) has an essential role in heterochromatin formation and mitotic progression through its interaction with various proteins. We have identified a unique HP1 alpha-binding protein, POGZ (pogo transposable element-derived protein with zinc finger domain), using an advanced proteomics approach. Proteins generally interact with HP1 through a PxVxL (where x is any amino-acid residue) motif; however, POGZ was found to bind to HP1 alpha through a zinc-finger-like motif. Binding by POGZ, mediated through its zinc-finger-like motif, competed with PxVxL proteins and destabilized the HP1 alpha-chromatin interaction. Depletion experiments confirmed that the POGZ HP1-binding domain is essential for normal mitotic progression and dissociation of HP1 alpha from mitotic chromosome arms. Furthermore, POGZ is required for the correct activation and dissociation of Aurora B kinase from chromosome arms during M phase. These results reveal POGZ as an essential protein that links HP1 alpha dissociation with Aurora B kinase activation during mitosis.
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