Journal
NATURE CELL BIOLOGY
Volume 11, Issue 8, Pages 1039-U283Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb1918
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Funding
- BBSRC
- MRC [G0601600]
- Wellcome Trust [GR077544AIA]
- MRC [G0601600] Funding Source: UKRI
- Medical Research Council [G0601600] Funding Source: researchfish
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Key features of clathrin-mediated endocytosis have been conserved across evolution. However, endocytosis in Saccharomyces cerevisiae is completely dependent on a functional actin cytoskeleton, whereas actin appears to be less critical in mammalian cell endocytosis. We reveal that the fundamental requirement for actin in the early stages of yeast endocytosis is to provide a strong framework to support the force generation needed to direct the invaginating plasma membrane into the cell against turgor pressure. By providing osmotic support, pressure differences across the plasma membrane were removed and this reduced the requirement for actin-bundling proteins in normal endocytosis. Conversely, increased turgor pressure in specific yeast mutants correlated with a decreased rate of endocytic patch invagination.
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