Journal
NATURE CELL BIOLOGY
Volume 10, Issue 11, Pages 1301-U101Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb1788
Keywords
-
Categories
Funding
- Stowers Institute for Medical Research
Ask authors/readers for more resources
Movement of meiosis I (MI) chromosomes from the oocyte centre to a subcortical location is the first step in the establishment of cortical polarity. this is required for two consecutive rounds of asymmetric meiotic cell divisions, which generate a mature egg and two polar bodies(1). Here we use live-cell imaging and genetic and pharmacological manipulations to determine the force-generating mechanism underlying this chromosome movement. Chromosomes were observed to move toward the cortex in a pulsatile manner along a meandering path. this movement is not propelled by myosin-II-driven cortical flow but is associated with a cloud of dynamic actin filaments trailing behind the chromosomes/spindle. Formation of these filaments depends on the actin nucleation activity of Fmn2, a formin-family protein that concentrates around chromosomes through its amino-terminal region. symmetry breaking of the actin cloud relative to chromosomes, and net chromosome translocation toward the cortex require actin turnover.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available