Journal
NATURE BIOTECHNOLOGY
Volume 33, Issue 1, Pages 58-U230Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3070
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Funding
- National Heart, Lung, and Blood Institute (NHLBI) Progenitor Cell Biology Consortium [R01HL75737, U01HL107440, UO1-HL100001, U01HL87402, U01HL100408]
- National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) [R24DK092760]
- National Institute of Neurological Disorders and Stroke (NINDS) [PO1NS066888]
- SMA Foundation
- Jerome Le Jeune Foundation
- EMBO postdoctoral fellowship
- Harvard Stem Cell Institute
- NATIONAL CANCER INSTITUTE [R01CA054358] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [UL1RR025758] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL048801, U01HL107440, U01HL100408, U01HL100001] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R24DK092760, F32DK095537] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM107536] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [P01NS066888] Funding Source: NIH RePORTER
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Human induced pluripotent stem cells (hiPSCs(1-3)) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation of the most widely used techniques for generating integration-free hiPSCs. Here we compare Sendai-viral (SeV)(4), episomal (Epi)(5) and mRNA transfection mRNA(6) methods using a number of criteria. All methods generated high-quality hiPSCs, but significant differences existed in aneuploidy rates, reprogramming efficiency, reliability and workload. We discuss the advantages and shortcomings of each approach, and present and review the results of a survey of a large number of human reprogramming laboratories on their independent experiences and preferences. Our analysis provides a valuable resource to inform the use of specific reprogramming methods for different laboratories and different applications, including clinical translation.
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