Journal
NATURE BIOTECHNOLOGY
Volume 32, Issue 10, Pages 1026-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3002
Keywords
-
Categories
Funding
- Canadian Institute of Health Research [MOP-84524, MOP-119507, MOP-106538, CPG-127793]
- Natural Sciences and Engineering Research Council of Canada [CHRPJ 446379-13]
- US National Institutes of Health [5U01 HL100405]
- VistaGen Therapeutics, Inc.
Ask authors/readers for more resources
The epicardium supports cardiomyocyte proliferation early in development and provides fibroblasts and vascular smooth muscle cells to the developing heart. The epicardium has been shown to play an important role during tissue remodeling after cardiac injury, making access to this cell lineage necessary for the study of regenerative medicine. Here we describe the generation of epicardial lineage cells from human pluripotent stem cells by stage-specific activation of the BMP and WNT signaling pathways. These cells display morphological characteristics and express markers of the epicardial lineage, including the transcription factors WT1 and TBX18 and the retinoic acid-producing enzyme ALDH1A2. When induced to undergo epithelial-to-mesenchymal transition, the cells give rise to populations that display characteristics of the fibroblast and vascular smooth muscle lineages. These findings identify BMP and WNT as key regulators of the epicardial lineage in vitro and provide a model for investigating epicardial function in human development and disease.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available