Journal
NATURE BIOTECHNOLOGY
Volume 31, Issue 3, Pages 227-229Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.2501
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Funding
- US National Institutes of Health (NIH) [DP1 GM105378]
- NIH [R01 GM088040, P50 HG005550, K01 AG031300]
- Jim and Ann Orr Research Scholar Award
- Massachusetts General Hospital
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In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR) CRISPR-associated (Gas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator like effector nucleases.
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