Journal
NATURE BIOTECHNOLOGY
Volume 31, Issue 4, Pages 331-334Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.2539
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Funding
- US National Institutes of Health [U54 CA156734]
- NIH [3P30CA023100-25S8]
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Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by massively parallel analysis using DNA sequencing. We demonstrate the broad utility of the method for human proteins by identifying known and previously unidentified interacting partners of LYN kinase, patient autoantibodies, and the small-molecules gefitinib and dasatinib.
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