Journal
NATURE BIOTECHNOLOGY
Volume 31, Issue 2, Pages 135-141Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.2478
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Funding
- CAS [KSZD-EW-Z-005]
- Chinese 973 programs [2007CB815700]
- National Natural Science Foundation of China [30960246, 31260538]
- Shenzhen Municipal Government
- Yantian District local government of Shenzhen
- CAS-Max Planck Society Fellowship
- CAS
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We report the similar to 2.66-Gb genome sequence of a female Yunnan black goat. The sequence was obtained by combining short-read sequencing data and optical mapping data from a high-throughput whole-genome mapping instrument. The whole-genome mapping data facilitated the assembly of super-scaffolds >5x longer by the N50 metric than scaffolds augmented by fosmid end sequencing (scaffold N50 = 3.06 Mb, super-scaffold N50 = 16.3 Mb). Super-scaffolds are anchored on chromosomes based on conserved synteny with cattle, and the assembly is well supported by two radiation hybrid maps of chromosome 1. We annotate 22,175 protein-coding genes, most of which were recovered in the RNA-seq data of ten tissues. Comparative transcriptomic analysis of the primary and secondary follicles of a cashmere goat reveal 51 genes that are differentially expressed between the two types of hair follicles. This study, whose results will facilitate goat genomics, shows that whole-genome mapping technology can be used for the de novo assembly of large genomes.
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