Journal
NATURE BIOTECHNOLOGY
Volume 29, Issue 3, Pages 279-U147Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.1783
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Funding
- A.L.S.
- P2ALS
- NYSTEM
- National Institutes of Health (NIH) [1RC2 NS069395-01, 5T32GM007592]
- National Institute of Neurological Disorders and Stroke
- National Institute of Child Health and Development
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Human induced pluripotent stem cells (iPSCs) present exciting opportunities for studying development and for in vitro disease modeling. However, reported variability in the behavior of iPSCs has called their utility into question. We established a test set of 16 iPSC lines from seven individuals of varying age, sex and health status, and extensively characterized the lines with respect to pluripotency and the ability to terminally differentiate. Under standardized procedures in two independent laboratories, 13 of the iPSC lines gave rise to functional motor neurons with a range of efficiencies similar to that of human embryonic stem cells (ESCs). Although three iPSC lines were resistant to neural differentiation, early neuralization rescued their performance. Therefore, all 16 iPSC lines passed a stringent test of differentiation capacity despite variations in karyotype and in the expression of early pluripotency markers and transgenes. This iPSC and ESC test set is a robust resource for those interested in the basic biology of stem cells and their applications.
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