Journal
NATURE BIOTECHNOLOGY
Volume 29, Issue 1, Pages 79-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.1720
Keywords
-
Categories
Funding
- Howard Hughes Medical Institute
- Starr Foundation
- Don Monti Memorial Research Foundation
- National Health and Medical Research Council of Australia
- NATIONAL CANCER INSTITUTE [P01CA013106, P30CA008748] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Short hairpin RNAs (shRNAs) are versatile tools for analyzing loss-of-function phenotypes in vitro and in vivo(1). However, their use for studying genes involved in proliferation and survival, which are potential therapeutic targets in cancer and other diseases, is confounded by the strong selective advantage of cells in which shRNA expression is inefficient. We therefore developed a toolkit that combines Tet-regulated miR30-shRNA technology, robust transactivator expression and two fluorescent reporters to track and isolate cells with potent target knockdown. We demonstrated that this system improves the study of essential genes and was sufficiently robust to eradicate aggressive cancer in mice by suppressing a single gene. Further, we applied this system for in vivo negative-selection screening with pooled shRNAs and propose a streamlined, inexpensive workflow that will facilitate the use of RNA interference (RNAi) for the identification and evaluation of essential therapeutic targets.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available