Journal
NATURE BIOTECHNOLOGY
Volume 28, Issue 6, Pages 581-583Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.1631
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Funding
- University of Michigan
- National Science Foundation (NSF)
- US National Institutes of Health [P20 GM-069985, R01 DE016530]
- National Institute of Dental and Craniofacial Research [T32]
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [R01DE016530, T32DE007057] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P20GM069985] Funding Source: NIH RePORTER
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We report a fully defined synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), which sustains long-term human embryonic stem (hES) cell growth in several different culture media, including commercially available defined media. The development of a standardized, controllable and sustainable culture matrix for hES cells is an essential step in elucidating mechanisms that control hES cell behavior and in optimizing conditions for biomedical applications of hES cells.
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