Journal
NATURE BIOTECHNOLOGY
Volume 27, Issue 12, Pages 1173-1175Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.1589
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Funding
- Burroughs Wellcome Fund
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We present a method that harnesses massively parallel DNA synthesis and sequencing for the high-throughput functional analysis of regulatory sequences at single-nucleotide resolution. As a proof of concept, we quantitatively assayed the effects of all possible single-nucleotide mutations for three bacteriophage promoters and three mammalian core promoters in a single experiment per promoter. The method may also serve as a rapid screening tool for regulatory element engineering in synthetic biology.
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