Journal
NATURE BIOTECHNOLOGY
Volume 26, Issue 10, Pages 1146-1153Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.1495
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Funding
- NHGRI NIH HHS [R21 HG004383, R01 HG003703-04, R01 HG003703, R21 HG004764-01, R01 HG003703-03, R01 HG003583] Funding Source: Medline
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R21HG004764, R01HG003703, R21HG004383, R01HG003583] Funding Source: NIH RePORTER
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A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of 'third generation' instruments that will sequence a diploid mammalian genome for similar to$1,000 in similar to 24 h.
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