Journal
NATURE BIOTECHNOLOGY
Volume 26, Issue 10, Pages 1179-1186Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.1500
Keywords
-
Categories
Funding
- National Institutes of Health (NIH) Metabolomics Roadmap initiative [AI068678]
- NIH [CA82396, CA85786]
- NIH Center for Systems Biology at Princeton University [5 P50 GM071508]
- National Science Foundation Faculty Early Career Development award program [MCB-0643859]
- Beckman Foundation
- American Heart Association [0635188N]
- National Science Foundation Dynamic Data-Driven Applications Systems program [CNS-0549181]
- NATIONAL CANCER INSTITUTE [R01CA085786, R01CA082396] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI078063] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P50GM071508] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Viruses rely on the metabolic network of their cellular hosts to provide energy and building blocks for viral replication. We developed a flux measurement approach based on liquid chromatography -tandem mass spectrometry to quantify changes in metabolic activity induced by human cytomegalovirus (HCMV). This approach reliably elucidated fluxes in cultured mammalian cells by monitoring metabolome labeling kinetics after feeding cells C-13-labeled forms of glucose and glutamine. Infection with HCMV markedly upregulated flux through much of the central carbon metabolism, including glycolysis. Particularly notable increases occurred in flux through the tricarboxylic acid cycle and its efflux to the fatty acid biosynthesis pathway. Pharmacological inhibition of fatty acid biosynthesis suppressed the replication of both HCMV and influenza A, another enveloped virus. These results show that fatty acid synthesis is essential for the replication of two divergent enveloped viruses and that systems-level metabolic flux profiling can identify metabolic targets for antiviral therapy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available