Journal
NATURE BIOTECHNOLOGY
Volume 26, Issue 3, Pages 305-312Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt1391
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Funding
- Biotechnology and Biological Sciences Research Council [BB/E025080/1] Funding Source: researchfish
- Medical Research Council [MC_U127527203, MC_U117532048] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/E025080/1] Funding Source: Medline
- Medical Research Council [MC_U127527203, MC_U117532048] Funding Source: Medline
- NHLBI NIH HHS [R33 HL073712] Funding Source: Medline
- NIDDK NIH HHS [U01 DK063630, DK63630, U01 DK063483, DK63328, R01 DK063400, U01 DK063328, R01 DK079798, DK63483, DK63481, R01 DK079798-01A2, DK63400, U01 DK063481] Funding Source: Medline
- BBSRC [BB/E025080/1] Funding Source: UKRI
- MRC [MC_U117532048, MC_U127527203] Funding Source: UKRI
Ask authors/readers for more resources
One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal.
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