Journal
NATURE BIOTECHNOLOGY
Volume 26, Issue 9, Pages 1015-1017Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.1491
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Funding
- Max-Planck Society
- EU
- BMBF
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Besides the well-documented integration of DNA flanked by the transfer DNA borders, occasional insertion of fragments from the tumor-inducing plasmid into plant genomes has also been reported during Agrobacterium tumefaciens-mediated transformation. We demonstrate that large (up to similar to 18 kb) gene-bearing fragments of Agrobacterium chromosomal DNA (AchrDNA) can be integrated into Arabidopsis thaliana genomic DNA during transformation. One in every 250 transgenic plants may carry AchrDNA fragments. This has implications for horizontal gene transfer and indicates a need for greater scrutiny of transgenic plants for undesired bacterial DNA.
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