Journal
NATURE
Volume 509, Issue 7501, Pages 487-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/nature13166
Keywords
-
Categories
Funding
- National Basic Research Program of China [2010CB911800]
- National Science Foundation of China [NSFC31170126, NSFC31070115]
- Peking-Tsinghua Centre for Life Sciences
Ask authors/readers for more resources
Targeted genome editing technologies are powerful tools for studying biology and disease, and have a broad range of research applications(1-7). In contrast to the rapid development of toolkits to manipulate individual genes, large-scale screening methods based on the complete loss of gene expression are only now beginning to be developed(8,9). Here we report the development of a focused CRISPR/Cas-based (clustered regularly interspaced short palindromic repeats/CRISPR-associated) lentiviral library in human cells and a method of gene identification based on functional screening and high-throughput sequencing analysis. Using knockout library screens, we successfully identified the host genes essential for the intoxication of cells by anthrax and diphtheria toxins, which were confirmed by functional validation. The broad application of this powerful genetic screening strategy will not only facilitate the rapid identification of genes important for bacterial toxicity but will also enable the discovery of genes that participate in other biological processes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available