Journal
NATURE
Volume 505, Issue 7484, Pages 564-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nature12819
Keywords
-
Categories
Funding
- Cancer Research UK
- Biotechnology and Biological Sciences Research Council [BB/K017438/1]
- BBSRC [BB/K017438/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/K017438/1] Funding Source: researchfish
- Cancer Research UK [17001, 10827] Funding Source: researchfish
- Novo Nordisk Fonden [NNF13OC0006477] Funding Source: researchfish
- Novo Nordisk Foundation Center for Protein Research [PI Michael Lund Nielsen] Funding Source: researchfish
Ask authors/readers for more resources
Nucleosomes are decorated with numerous post-translational modifications capable of influencing many DNA processes(1). Here we describe a new class of histone modification, methylation of glutamine, occurring on yeast histone H2A at position 105 (Q105) and human H2A at Q104. We identify Nop1 as the methyltransferase in yeast and demonstrate that fibrillarin is the orthologue enzyme in human cells. Glutamine methylation of H2A is restricted to the nucleolus. Global analysis in yeast, using an H2AQ105me-specific antibody, shows that this modification is exclusively enriched over the 35S ribosomal DNA transcriptional unit. We show that the Q105 residue is part of the binding site for the histone chaperone FACT (facilitator of chromatin transcription) complex(2). Methylation of Q105 or its substitution to alanine disrupts binding to FACT in vitro. A yeast strain mutated at Q105 shows reduced histone incorporation and increased transcription at the ribosomal DNA locus. These features are phenocopied by mutations in FACT complex components. Together these data identify glutamine methylation of H2A as the first histone epigenetic mark dedicated to a specific RNA polymerase and define its function as a regulator of FACT interaction with nucleosomes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available