Journal
NATURE
Volume 494, Issue 7438, Pages 480-483Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nature11897
Keywords
-
Categories
Funding
- National Institute of General Medical Sciences [GM26875]
Ask authors/readers for more resources
Biologists have long been concerned about what constrains variation in cell size, but progress in this field has been slow and stymied by experimental limitations'. Here we describe a new method, ergodic rate analysis (ERA), that uses single-cell measurements of fixed steady-state populations to accurately infer the rates of molecular events, including rates of cell growth. ERA exploits the fact that the number of cells in a particular state is related to the average transit time through that state(2). With this method, it is possible to calculate full time trajectories of any feature that can be labelled in fixed cells, for example levels of phosphoproteins or total cellular mass. Using ERA we find evidence for a size-discriminatory process at the G1/S transition that acts to decrease cell-to-cell size variation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available