Journal
NATURE
Volume 493, Issue 7430, Pages 56-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nature11801
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Funding
- Ministry of Science and Technology [2009CB918801]
- National Natural Science Foundation of China [30888001]
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Presenilin and signal peptide peptidase (SPP) are intramembrane aspartyl proteases that regulate important biological functions in eukaryotes. Mechanistic understanding of presenilin and SPP has been hamperedby lack of relevant structural information. Here we report the crystal structure of a presenilin/SPP homologue (PSH) from the archaeon Methanoculleus marisnigri JR1. The protease, comprising nine transmembrane segments (TMs), adopts a previously unreported protein fold. The amino-terminal domain, consisting of TM1-6, forms a horseshoe-shaped structure, surrounding TM7-9 of the carboxy-terminal domain. The two catalytic aspartate residues are located on the cytoplasmic side of TM6 and TM7, spatially close to each other and approximately 8 angstrom into the lipid membrane surface. Water molecules gain constant access to the catalytic aspartates through a large cavity between the amino-and carboxy-terminal domains. Structural analysis reveals insights into the presenilin/SPP family of intramembrane proteases.
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