Journal
NATURE
Volume 463, Issue 7282, Pages 808-U120Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nature08750
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Funding
- National Institutes of Health
- CRI-Irvington Institute
- CRI
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Immune homeostasis is dependent on tight control over the size of a population of regulatory T (T-reg) cells capable of suppressing over-exuberant immune responses. The T-reg cell subset is comprised of cells that commit to the T-reg lineage by upregulating the transcription factor Foxp3 either in the thymus (tT(reg)) or in the periphery (iT(reg))(1,2). Considering a central role for Foxp3 in T-reg cell differentiation and function(3,4), we proposed that conserved non-coding DNA sequence (CNS) elements at the Foxp3 locus encode information defining the size, composition and stability of the T-reg cell population. Here we describe the function of three Foxp3 CNS elements (CNS1-3) in T-reg cell fate determination in mice. The pioneer element CNS3, which acts to potently increase the frequency of T-reg cells generated in the thymus and the periphery, binds c-Rel in in vitro assays. In contrast, CNS1, which contains a TGF-beta-NFAT response element, is superfluous for tT(reg) cell differentiation, but has a prominent role in iT(reg) cell generation in gut-associated lymphoid tissues. CNS2, although dispensable for Foxp3 induction, is required for Foxp3 expression in the progeny of dividing T-reg cells. Foxp3 binds to CNS2 in a Cbf-beta-Runx1 and CpG DNA demethylation-dependent manner, suggesting that Foxp3 recruitment to this 'cellular memory module' facilitates the heritable maintenance of the active state of the Foxp3 locus and, therefore, T-reg lineage stability. Together, our studies demonstrate that the composition, size and maintenance of the T-reg cell population are controlled by Foxp3 CNS elements engaged in response to distinct cell-extrinsic or -intrinsic cues.
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