Journal
NATURE
Volume 453, Issue 7193, Pages 358-U27Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nature06929
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Funding
- NHGRI NIH HHS [R01 HG004160-01A1, HG004160, R01 HG004160] Funding Source: Medline
- NIGMS NIH HHS [GM47477, R01 GM047477] Funding Source: Medline
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Comparative genomics of nucleosome positions provides a powerful means for understanding how the organization of chromatin and the transcription machinery co-evolve. Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from the yeast Saccharomyces cerevisiae. Like Saccharomyces, Drosophila nucleosomes are organized around active transcription start sites in a canonical -1, nucleosome-free region, +1 arrangement. However, Drosophila does not incorporate H2A.Z into the -1 nucleosome and does not bury its transcriptional start site in the +1 nucleosome. At thousands of genes, RNA polymerase II engages the +1 nucleosome and pauses. How the transcription initiation machinery contends with the +1 nucleosome seems to be fundamentally different across major eukaryotic lines.
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