Journal
NATURAL PRODUCT RESEARCH
Volume 29, Issue 8, Pages 745-749Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2014.986657
Keywords
shoot biomass; elicitation; Brahmi; HPLC; secondary metabolite production
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Funding
- UGC, GOI [F. 7-324/2011(BSR)]
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The effect of different abiotic elicitors [jasmonic acid, copper sulphate (CuSO4) and salicyclic acid] at varying concentrations on the stimulation of biomass and bacoside production in in vitro Bacopa monnieri shoot culture was studied. A systematic study conducted over a period of 35 days indicated that the maximum bacoside production (6.74mgg(-1) dry weight (DW)) was obtained after a lag of 7 days and thereafter, the content decreased gradually to again increase at 28 days (5.91mgg(-1) DW). Therefore, elicitation experiments were carried out over a period of 3, 6 and 9 days. The shoot cultures treated with 45mgL(-1) of CuSO4 exhibited the highest bacoside content of 8.73mgg(-1) DW (similar to 1.42-fold higher) than in control cultures (6.14mgg(-1) DW). This study indicates the effectiveness of abiotic elicitation on bacoside production in in vitro shoot cultures of this medicinally important herb known for its memory-enhancing properties.
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