Journal
NATURAL PRODUCT RESEARCH
Volume 25, Issue 4, Pages 332-340Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786411003752086
Keywords
macranthoside B; HL-60 cells; apoptosis; caspase-3; membrane permeabilisation; cholesterol
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Funding
- Jiangsu Provincial Natural Science Foundation of China [BK2008353]
- Jiangsu Center for Research and Development of Medicinal Plants [2008001]
- National Key Technologies R & D Program of China [2009ZX09103-397]
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Triterpene saponins are throught to be potential anti-tumour agents in many cell types. This study aims to evaluate the cytotoxic activity and mechanism of a triterpene saponin, macranthoside B (MB), isolated from Lonicera macranthoides Hand.-Mazz. (Caprifoliaceae). A cell viability assay showed that MB inhibited cell growth of a panel of six cancer cell lines, especially in human acute promyelocytic leukaemia HL-60 cells, with an IC50 value of 3.8 mu mol. A hypodiploid cells assay and an annexin-V-FITC/PI double staining assay showed a significant increase of apoptosis in a dose-dependent manner on HL-60 cells both 24 and 48 h after MB treatment. MB-induced apoptosis was through the caspase-mediated pathway, by activation of caspase-3. Furthermore, a lactate dehydrogenase (LDH) release test suggested that an MB-cholesterol interaction led to the rearrangement of the lipid bilayer and to subsequent cell membrane impairment. Taken together, these findings demonstrate that MB may exhibit cytotoxic activity against HL-60 cells by inducing apoptosis via caspase-dependent pathways and also membrane permeabilisation.
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