Antifungal activity of bioactive triterpenoid saponin from the seeds of Cassia angustifolia

A novel oleanen type triterpenoid glycoside has been isolated from butanolic seed extracts of Cassia angustifolia. Its structure was elucidated as 3-O-{-D-glucuronopyranosyl-(1 4)-[-D-galactopyranosyl-(1 2)]--D-xylopyranosyl-(1 3)--D-glucopyranosyl}-2, 16-dihydroxy-4, 20-hydroxy methyl olean-12-ene-28-oic acid on the basis of spectral evidence (i.e. FTIR, 1H NMR, 13C NMR and FAB-MS data). The isolated saponin was tested for its antifungal activity, where the maximum inhibition was found in Colletotrichium dematium.