Journal
NATURAL PRODUCT REPORTS
Volume 35, Issue 11, Pages 1210-1228Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8np00036k
Keywords
-
Funding
- Health Research Council of New Zealand [16/172]
- Rutherford Discovery Fellowship - Royal Society of New Zealand
- Victoria Doctoral Scholarship
- Victoria University Postgraduate Research Excellence Award
- Victoria Submission Scholarship
Ask authors/readers for more resources
Covering: up to May 2018Non-ribosomal peptide synthetases (NRPSs) are mega-enzymes that form modular templates to assemble specific peptide products, independent of the ribosome. The autonomous nature of the modules in the template offers prospects for re-engineering NRPS enzymes to generate modified peptide products. Although this has clearly been a primary mechanism of natural product diversification throughout evolution, equivalent strategies have proven challenging to implement in the laboratory. In this review we examine key examples of successful and less-successful re-engineering of NRPS templates to generate novel peptides, with the aim of extracting practical guidelines to inform future efforts. We emphasise the importance of maintaining effective protein-protein interactions in recombinant NRPS templates, and identify strengths and limitations of diverse strategies for achieving different engineering outcomes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available