Journal
NANOTOXICOLOGY
Volume 7, Issue 5, Pages 935-952Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/17435390.2012.676098
Keywords
In vitro; nanoparticles; oxidative stress; ICP-MS; LUCS
Categories
Funding
- Spanish Ministerio de Medio Ambiente y Medio Rural y Marino [AEG07-060]
- FP7-PEOPLE-ITN ECO (Environmental ChemOinformatics) [238701]
Ask authors/readers for more resources
The increasing presence of ZnO nanoparticles (NPs) in consumer products may be having a dramatic impact in aquatic environments. The evaluation of ZnO NP toxicity represents a great challenge. This study aimed at evaluating the cytotoxic effect of micro-and nanosized ZnO in a fish and a mammalian hepatoma cell line. A detailed characterisation of the particles in exposure media showed that ZnO NPs formed large aggregates. ZnO cytotoxicity was evaluated with a battery of in vitro assays including LUCS, a new approach based on DNA alteration measurements. In fish cells, ZnO NP aggregates contributed substantially to the cytotoxic effects whereas toxicity in the human cells appeared to be mainly produced by the dissolved fraction. ROS production did not contribute to the observed cytotoxicity. This work also showed that measuring concentrations of NPs is essential to understand the mechanisms underlying their toxicity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available