Journal
NANOTOXICOLOGY
Volume 7, Issue 4, Pages 417-431Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/17435390.2012.666576
Keywords
Nanotoxicology; liquid suspension; DLS; dosimetry
Categories
Funding
- NIEHS [ES-0000002]
- Center for Nanotechnology and Nanotoxicology at The Harvard School of Public Health
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1235806] Funding Source: National Science Foundation
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In vitro toxicity assays are efficient and inexpensive tools for screening the increasing number of engineered nanomaterials (ENMs) entering the consumer market. However, the data produced by in vitro studies often vary substantially among different studies and from in vivo data. In part, these discrepancies may be attributable to lack of standardisation in dispersion protocols and inadequate characterisation of particle-media interactions which may affect the particle kinetics and the dose delivered to cells. In this study, a novel approach for preparation of monodisperse, stabilised liquid suspensions is presented and coupled with a numerical model which estimates delivered dose values. Empirically derived material-and media-specific functions are presented for each media-ENM system that can be used to convert administered doses to delivered doses. The interactions of ENMs with a variety of physiologic media were investigated and the importance of this approach was demonstrated by in vitro cytotoxicity assays using THP-1 macrophages.
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