Journal
NANOTOXICOLOGY
Volume 7, Issue 4, Pages 432-440Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/17435390.2012.668569
Keywords
Nanoparticles; soil organisms; histology; gene expression; enzyme activity
Categories
Funding
- Wageningen UR strategic research program BioNanotechnology
- research school WIMEK
Ask authors/readers for more resources
Effects of C-60 exposure (0, 15 or 154 mg/kg soil) on the earthworm Lumbricus rubellus were assessed at the tissue and molecular level, in two experiments. In the first experiment, earthworms were exposed for four weeks, and in the second lifelong. In both experiments, gene expression of heat shock protein 70 (HSP70) decreased. For catalase and glutathione-S-transferase (GST), no significant trends in gene expression or enzyme activity were observed. Gene expression of coelomic cytolytic factor-1 (CCF-1) did not alter in earthworms exposed for four weeks, but was significantly down-regulated in the lifelong exposure. Histology of earthworms exposed to C-60 in both experiments showed a damaged cuticle, with underlying pathologies of epidermis and muscles, as well as effects on the gut barrier. However, tissue repair was also observed in these earthworms. Overall, these data show that sub-lethal C-60 exposure to earthworms via the soil affects gene expression and causes tissue pathologies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available